abstract：:The effects of cholesterol oxides on regular and neuronal PC12 cells were examined. Among the cholesterol oxides tested, 7-beta-, 22(R)- and 25-OH-cholesterol were very toxic to regular PC12 cells such that approximately 80% of cells were killed after a 3 day incubation with 20 micrograms/ml (50 microM) of these agent...

journal_title：Neurochemistry international

authors： Chang JY,Liu LZ

更新日期：1997-10-01 00:00:00

abstract：:This report describes the effects of endothelins (ET-1 and ET-3) on ion transport systems expressed on cultured rat brain capillary endothelial cells (RBEC) and includes investigation of pharmacological properties of ET receptors, their reactivity and induction of signal transduction pathways. ET-1 stimulated IP3 form...

journal_title：Neurochemistry international

authors： Kawai N,Yamamoto T,Yamamoto H,McCarron RM,Spatz M

更新日期：1997-10-01 00:00:00

abstract：:The use of synthetic antisense oligonucleotides as specific inhibitors of gene expression exploits the susceptibility of mRNA to functional blockade at several levels, including mRNA processing, transport, translation and degradation. It is becoming increasingly apparent that the actions of these synthetic oligomers a...

journal_title：Neurochemistry international

authors： Knee R,Murphy PR

更新日期：1997-09-01 00:00:00

abstract：:Cerebellar granule neurons produce homogenous cultures that provide a unique opportunity for quantifying the apoptosis by propidium iodide- and deoxynucleotidyl transferase-flow cytometry combined analysis and for studying its regulation by neurotrophins. Nerve growth factor (NGF) was found to promote postmitotic surv...

journal_title：Neurochemistry international

authors： Muller Y,Tangre K,Clos J

更新日期：1997-08-01 00:00:00

abstract：:To identify whether the process of apoptosis bears a topographic relationship to selected aspects of Alzheimer's disease (AD) pathology, we used an in situ nick translation method (TUNEL) to map DNA fragmentation in hippocampal sections immunostained for abnormally phosphorylated tau, which exists in the neurofibrilla...

journal_title：Neurochemistry international

authors： Sugaya K,Reeves M,McKinney M

更新日期：1997-08-01 00:00:00

abstract：:A single molecular species of GTP-binding protein (G protein) has been purified from the bovine cerebral cortex. The immunoblot analysis indicated that the isolated G protein might be Gi1 or Gi2 but not Go, since it was reacted by specific antibodies, anti-Gi alpha 1-2 and anti-Gi alpha 1-1, but not anti-Go alpha. Whe...

journal_title：Neurochemistry international

authors： Nishikawa M,Hirouchi M,Kuriyama K

更新日期：1997-07-01 00:00:00

abstract：:In chicks submitted to light stimulation, the synthesis of gangliosides of the retina ganglion cell increases with respect to chicks maintained in the dark. In an attempt to elucidate if the activation of glycosyltransferases participates in the establishment of these light-dark differences detected in vivo, we examin...

journal_title：Neurochemistry international

authors： Bussolino DF,Guido ME,Caputto BL

更新日期：1997-07-01 00:00:00

abstract：:Structural analogues of ZAPA, Z-3-[(aminoiminomethyl)thio]prop-2-enoic acid, an isothiouronium analogue of GABA, are potent GABAA agonists as seen in the isolated guinea-pig ileum and in the facilitation of [3H]diazepam binding to rat brain membranes. Compounds with guanidino or amidine groups replacing the amino func...

journal_title：Neurochemistry international

authors： Allan RD,Dickenson HW,Johnston GA,Kazlauskas R,Mewett KN

更新日期：1997-06-01 00:00:00

abstract：:Cytokines are a heterogenous group of polypeptide mediators that have been associated with activation of numerous functions, including the immune system and inflammatory responses. The cytokine families include, but are not limited to, interleukins (IL-I alpha, IL-I beta, ILIra and IL-2-IL-15), chemokines (IL-8/ NAP-I...

journal_title：Neurochemistry international

authors： Ebadi M,Bashir RM,Heidrick ML,Hamada FM,Refaey HE,Hamed A,Helal G,Baxi MD,Cerutis DR,Lassi NK

更新日期：1997-04-01 00:00:00

abstract：:Interferon beta-1b (IFN beta-1b) (Betaseron) has been recently shown to alter the course of multiple sclerosis (MS), an inflammatory demyelinating disease of the central nervous system (CNS) where migration of activated lymphocytes across the blood-brain barrier (BBB) is a critical step in the pathogenesis of this dis...

journal_title：Neurochemistry international

authors： Jiang H,Williams GJ,Dhib-Jalbut S

更新日期：1997-04-01 00:00:00

abstract：:The presence of corticotropin-releasing hormone (CRH) receptors has been previously demonstrated in corticotrophs from normal pituitaries using a method combining immunocytochemistry and liquid emulsion autoradiography. The aim of this study was to compare the characteristics of the 125I-Tyr0-hCRH binding in corticotr...

journal_title：Neurochemistry international

authors： Abs R,Smets G,Vauquelin G,Verhelst J,Mahler C,Verlooy J,Stevenaert A,Wouters L,Borgers M,Beckers A

更新日期：1997-03-01 00:00:00

abstract：:The protein kinase C (PKC) family of serine/threonine kinase isoenzymes are universally expressed in vertebrate tissues where they control vital cellular functioning. PKC comprises twelve currently identified mammalian isoenzymes, described in three distinct groups according to their need for different effector stimul...

journal_title：Neurochemistry international

authors： Wood JP,McCord RJ,Osborne NN

更新日期：1997-02-01 00:00:00

abstract：:Long-term potentiation (LTP), a model of activity-dependent synaptic plasticity and of certain forms of memory, comprises the persistent enhancement of excitatory neurotransmission that results from high-frequency activation. A presynaptic component of LTP is thought to be modulated by a retrograde messenger generated...

journal_title：Neurochemistry international

authors： Bazan NG,Packard MG,Teather L,Allan G

更新日期：1997-02-01 00:00:00

abstract：:A low density of brain IR2-imidazoline receptive sites has previously been linked to depression. In this study we evaluated brain IR2-binding sites in a rat model of depression, olfactory bulbectomy, and determined the effects of chronic imipramine treatment in vivo on these sites. Compared with sham-operated controls...

journal_title：Neurochemistry international

authors： Zhu H,Paul IA,McNamara M,Redmond A,Nowak G,Piletz JE

更新日期：1997-01-01 00:00:00

abstract：:The present study was carried out to clarify whether the imidazolines clonidine, moxonidine and cirazoline as well as the guanidine aganodine inhibit noradrenaline release in the rat and rabbit brain via imidazoline receptors, alpha 2-adrenoceptors and/or histamine H3 receptors. Slices or synaptosomes from the rat or ...

journal_title：Neurochemistry international

authors： Schlicker E,Fink K,Kathmann M,Molderings GJ,Göthert M

更新日期：1997-01-01 00:00:00

abstract：:Acetylcholinesterase release in the guinea-pig substantia nigra has been previously investigated 'on-line', using a sensitive chemiluminescent system. Since histological observations suggest that there is a difference in acetylcholinesterase distribution in the rat substantia nigra compared to that of the guinea-pig, ...

journal_title：Neurochemistry international

authors： Dally JJ,Schaefer M,Greenfield SA

更新日期：1996-12-01 00:00:00

abstract：:We have previously reported that, unlike their rat counterparts, the survival of mouse cerebellar granule cells is independent of chronic stimulation whether owing to elevated K(+)-induced depolarization or NMDA (N-methyl-D-aspartate) receptor activation. One explanation could be that during the critical period mouse ...

journal_title：Neurochemistry international

authors： Mogensen HS,Jørgensen OS

更新日期：1996-11-01 00:00:00

abstract：:We tested the ability of the non-competitive N-methyl-D-aspartate (NMDA) antagonist, dizocilpine (MK-801), to promote protection against the acute effect of 1-methyl-4-phenylpyridinium ion (MPP+) in rat striatal slices. Pretreatment with MK-801 at concentrations higher than 15 microM partially prevented the dopamine (...

journal_title：Neurochemistry international

authors： Venero JL,Santiago M,Machado A,Cano J

更新日期：1996-10-01 00:00:00

abstract：:The excitatory action of glutamate and aspartate upon direct application to the motor cortex of dogs was first reported in 1952 by Takashi Hayashi, Keio University School of Medicine, Tokyo. Although this investigation had been performed in the early 1940s during the Second World War, the publication in English was de...

journal_title：Neurochemistry international

authors： Takagaki G

更新日期：1996-09-01 00:00:00

abstract：:(RS)-2-Amino-3-(3-hydroxy-5-phenyl-4-isoxazolyl)propionic acid (APPA), which is an analogue of (RS)-2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid (AMPA), shows the characteristics of a partial AMPA receptor agonist. Since (S)-APPA is a full AMPA agonist and (R)-APPA a competitive antagonist, the partial ag...

journal_title：Neurochemistry international

authors： Ebert B,Madsen U,Søby KK,Krogsgaard-Larsen P

更新日期：1996-09-01 00:00:00

abstract：:The concentrations of gamma-glutamylglutamate, gamma-glutamylglutamine, gamma-glutamylcysteine, glutamate, aspartate, glutamine, cyst(e)ine and glutathione (including disulfides) were determined by HPLC analysis of both the tissue and the surrounding medium of incubated rat hippocampal slices. High potassium concentra...

journal_title：Neurochemistry international

authors： Li X,Orwar O,Revesjö C,Sandberg M

更新日期：1996-08-01 00:00:00

abstract：:Free radicals have been implicated in the pathogenesis of vasogenic brain edema caused by ischemic or traumatic injury. It has been reported that in transgenic mice overexpressing the human CuZn-superoxide dismutase, brain edema is decreased in many cerebral disorders. To investigate the effects of free radicals on th...

journal_title：Neurochemistry international

authors： Imaizumi S,Kondo T,Deli MA,Gobbel G,Joó F,Epstein CJ,Yoshimoto T,Chan PH

更新日期：1996-08-01 00:00:00

abstract：:Thromboxane A2(TxA2) is a potent vasoconstrictor associated with cerebrovascular disease and is thought to be synthesized within tissues of the brain. In order to determine the cellular sources of TxA2 in the central nervous system (CNS), we measured the release of the stable metabolite TxB2 in cultures of mixed or hi...

journal_title：Neurochemistry international

authors： Giulian D,Corpuz M,Richmond B,Wendt E,Hall ER

更新日期：1996-07-01 00:00:00

abstract：:Biochemical abnormalities have been implicated in possible mechanisms underlying the epileptic phenomena. Some of these alterations include changes in the activity of several enzymes present in epileptic tissues. Systemic administration of pilocarpine in rats induces electrographic and behavioral limbic seizures and s...

journal_title：Neurochemistry international

authors： Fernandes MJ,Naffah-Mazzacoratti MG,Cavalheiro EA

更新日期：1996-05-01 00:00:00

abstract：:The binding kinetics of the specific dopamine D2 antagonist [3H]raclopride to dopamine D2 receptors in rat neostriatum were studied. The pseudo-first-order rate constants of [3H]raclopride binding with these membranes revealed a hyperbolic dependence upon the antagonist concentration, indicating that the reaction had ...

journal_title：Neurochemistry international

authors： Lepiku M,Rinken A,Järv J,Fuxe K

更新日期：1996-05-01 00:00:00

abstract：:The observation that autophosphorylation converts CaM kinase II from the Ca(2+)-dependent form to the Ca(2+)-independent form has led to speculation that the formation of the Ca(2+)-independent form of the enzyme could encode frequency of synaptic usage and serve as a molecular explanation of "memory". In cultured rat...

journal_title：Neurochemistry international

authors： Fukunaga K,Muller D,Miyamoto E

更新日期：1996-04-01 00:00:00

abstract：:A significant increase in the number of measurable [3H]Ro 5-4864 receptors was found in forebrain membranes of chicks submitted to 15 min of acute swim stress compared to non-stressed chicks. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]Ro 5-4864 r...

journal_title：Neurochemistry international

authors： Marín RH,Arce A,Martijena ID

更新日期：1996-04-01 00:00:00

abstract：:Conventional kinetic models of brain glucose uptake and metabolism that visualize brain glucose as being in a single pool in equilibrium with plasma, are unable to account for some recently described experimental findings. These include microdialysis demonstrations of a brain extracellular fluid glucose concentration ...

journal_title：Neurochemistry international

authors： Forsyth RJ

更新日期：1996-03-01 00:00:00

abstract：:The effect of 1-methyl-isoquinoline (MIQ+) on the respiratory inhibition and the uptake of MIQ+ were measured using mouse liver mitochondria. MIQ+ inhibited the electron transport of complex I but did not inhibit the respiration of mitochondria with succinate as a substrate. MIQ+ was taken up by mitochondria in an ene...

journal_title：Neurochemistry international

authors： Aiuchi T,Matsunaga M,Syo M,Nakaya K

更新日期：1996-03-01 00:00:00

abstract：:Succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide (Suc-LLVY-MCA) hydrolyzing activities of the 20S and 26S proteasomes in the gerbil cortex following transient forebrain ischemia were examined. Using extraction solutions without ATP, only 20S proteasome activity was noted after separation with glycerol gradient centri...

journal_title：Neurochemistry international

authors： Kamikubo T,Hayashi T

更新日期：1996-02-01 00:00:00

abstract：:The effects of N-methyl-norsalsolinol on basal and inhibited activity of monoamine oxidase (MAO) in membrane preparations from the caudate nucleus of the rat have been studied. For the first time, the physiological step of dopamine oxidation to 3, 4 dihydroxyphenylacetic aldehyde (DPAA) was examined. N-methyl-norsalso...

journal_title：Neurochemistry international

authors： Moser A,Scholz J,Bamberg H,Böhme V

更新日期：1996-01-01 00:00:00

abstract：:Taurine-like immunoreactivity (TLI) is found in the pigeon retina at high levels within cones, in select cells of the inner nuclear layer and in sublaminae of the inner plexiform layer. At the ultrastructural level a high density of immuno-gold particles in visualized in the ellipsoid, but not in the paraboloid area o...

journal_title：Neurochemistry international

authors： Ueck M,Hach A,Verdone-Smith C,Lake N

更新日期：1995-12-01 00:00:00

abstract：:Outer (OHC) and inner (IHC) hair cells in the organ of Corti of the mammalian cochlea process sound. OHC and their efferent synapse are part of a feedback system assumed to control and modulate information carried by afferent neurons passing from IHC to the brain. Underlying mechanisms are not well understood. This pa...

journal_title：Neurochemistry international

authors： Heilbronn E,Järlebark L,Lawoko G

更新日期：1995-10-01 00:00:00

abstract：:In this study we determined the changes in the intracellular free Ca2+ concentration, associated with the inhibitory modulation of the exocytotic release of GABA by GABAB receptor activation in rat cerebrocortical synaptosomes. We observed that SK&F 97541 and (-)baclofen both act as agonists of the presynaptic GABAB r...

journal_title：Neurochemistry international

authors： Santos AE,Carvalho CM,Macedo TA,Carvalho AP

更新日期：1995-10-01 00:00:00

abstract：:Synaptic like microvesicles (SLMV) form a pleiomorphic population of vesicles present in neuroendocrine cells of the pituitary and the adrenal medulla. Their composition and biogenesis is very similar to small synaptic vesicles (SSV) present in nerve terminals. Membrane proteins of SSV involved in fusion (synaptotagmi...

journal_title：Neurochemistry international

authors： Llona I

更新日期：1995-09-01 00:00:00

abstract：:The roles of cyclic AMP and calcium in the regulation of serotonin N-acetyltransferase (NAT) activity were studied in low density monolayer cultures of chick retinal photoreceptors and neurons. Photoreceptor-enriched retinal cell cultures were prepared from embryonic day 6 retinas and cultured for 6 days. NAT activity...

journal_title：Neurochemistry international

authors： Gan J,Alonso-Gómez AL,Avendano G,Johnson B,Iuvone PM

更新日期：1995-08-01 00:00:00

abstract：:Immunohistochemical study on growth inhibitory factor (GIF) in rat brain has revealed that a glial cell layer on the surface of cerebral cortex and the cells surrounding Purkinje cells has been reported. In addition, neurons in gray matter were weakly immunostained for GIF. In situ hybridization using digoxigenin-labe...

journal_title：Neurochemistry international

authors： Anezaki T,Ishiguro H,Hozumi I,Inuzuka T,Hiraiwa M,Kobayashi H,Yuguchi T,Wanaka A,Uda Y,Miyatake T

更新日期：1995-07-01 00:00:00

abstract：:In the present study we have investigated the relationship between serotonergic receptor(s) (5-HTR) and muscarinic cholinergic receptor (mAChR) activation, leading to the arachidonic acid (AA) release and inositol phosphates (IPs) formation in adult and aged brain cortex synaptoneurosomes. It was observed that seroton...

journal_title：Neurochemistry international

authors： Samochocki M,Strosznajder J

更新日期：1995-06-01 00:00:00

abstract：:The kinetics of oxidation of endogenous antioxidants such as vitamins C and E and thiols as well as membrane cholesterol in isolated rat brain mitochondria were studied. Oxidation was induced by incubating the mitochondria at 37 degrees C with the free radical generators 2,2' azobis (2'-amidinopropane) dihydrochloride...

journal_title：Neurochemistry international

authors： Vatassery GT,Smith WE,Quach HT,Lai JC

更新日期：1995-05-01 00:00:00

abstract：:The mobilization of inositol triphosphate ip3 by N-methyl D-aspartate (NMDA) and kainate, two excitatory amino acid EAA receptor agonists, was studied in cultured chick retina cells as a function of culture differentiation. Kainate (EC50 = 30 microM) stimulated from 6 to 9-fold the production of [3H]ip3 between E8C3 (...

journal_title：Neurochemistry international

authors： Reis RA,Kubrusly RC,de Mello MC,de Mello FG

更新日期：1995-04-01 00:00:00